Part:BBa_K414001:Design

Strong Plant RBS (Kozak sequence) + GFP from E0040

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 662
  

Design Notes

We chose a strong plant Kozak sequence that can function in a broad range of dicot species, including tobacco and Arabidopsis.

Source

The Kozak sequence was directly engineered into the prefix primer and used to PCR out the GFP gene from E0040.